目錄
PART I Elements of Biochemistry and Molecular Biology Experiments
Chapter 1 Basic Requirements of Biochemistry and Molecular Biology Experiments
Section 1 The Validity of Biochemistry and Molecular Biology Experiments
Section 2 The Requirements for Experimental Records and Report
Section 3 Preparation of Experimental Samples
Section 4 Requirements for Glass Instruments Washing and Preparation Methods for Various Cleaning Solutions
Section 5 Precautions for Reagent Preparation
Section 6 Laboratory Safety and Protection
Chapter 2 Basic Principles and Techniques of Biochemistry and Molecular Biology
Section 1 Overview of Biological Molecules
Section 2 Ultraviolet-Visible Absorption Spectroscopy
Section 3 Centrifugation
Section 4 Chromatography Techniques
Section 5 Electrophoresis
Section 6 Genetic Engineering and Gene Expression Analysis Technologies
Chapter 3 Buffer Solutions
Section 1 Buffer Theory
Section 2 Common Buffer Preparation Methods
Section 3 Modernization and Optimization
Section 4 Buffer Use
PART II Experiments of The Biochemistry and Molecular Biology
Chapter 1 Biomacromolecules Experiments
Section 1 Determination of Hemoglobin Content by Spectrophotometry
Section 2 Determination of Protein Concentration
Section 3 Protein Denaturation, Precipitation, and Isoelectric Point Determination
Section 4 Serum Protein Electrophoresis on Cellulose Acetate Membrane
Section 5 Factors Influencing Enzyme Activity: Temperature, pH, Activators, and Inhibitors
Section 6 Determination of Km Value for Alkaline Phosphatase
Section 7 Hemoglobin Gel Filtration Chromatography
Section 8 Column Chromatography of Carotene
Chapter 2 Metabolism Experiments
Section 1 Determination of Serum Glucose
Section 2 Glycogenolysis of Muscle Glycogen
Section 3 Lactate Dehydrogenase and Coenzyme I
Section 4 Competitive Inhibition of Succinate Dehydrogenase
Section 5 Ketogenesis and Identification of Ketone Bodies
Section 6 Determination of Total Serum Cholesterol
Section 7 Determination of Serum Alanine Aminotransferase (ALT) Activity (King's Method)
Chapter 3 Experiment of Molecular Biology
Section 1 Extraction and Qualitative/Quantitative Analysis of Genomic DNA
Section 2 Extraction and ElectrophoreticAnalysis of Plasmid DNA
Section 3 RNA Extraction and Reverse Transcription
Section 4 Polymerase Chain Reaction (PCR)
Section 5 DNA Agarose Gel Electrophoresis
Section 6 Protein Extraction and Polyacrylamide Gel Electrophoresis
Section 7 Western Blot Analysis of Target Protein Expression Levels
Chapter 4 Open Experiment
Section 1 Extraction of DNA from Animal Hair for RAPD Amplification
Section 2 Gene Polymorphism Analysis
Section 3 DNA Fingerprinting Analysis
Section 4 Electrophoretic Mobility Shift Assay
PART III Commonly Used Methods and Precautions in Biochemistry and Molecular Biology Experiments
Chapter 1 Commonly Used Tools and Experimental Methods
Section 1 Common Tools for Nucleic Acid and Protein Sequence Data Analysis
Section 2 Nucleic Acid Staining Techniques
Section 3 Protein Staining
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