8.1 培養基的配製 8.2 滅菌、消毒與接種 8.3 繼代培養與擴繁 8.4 試管苗的馴化、移栽和管理 第9章 植物逆境生理 9.1 脯氨酸含量的測定 9.2 總黃酮含量的測定 9.3 抗壞血酸（AsA）含量的測定 9.4 谷胱甘?（GsH）含量的測定 9.5 細胞質膜透性的檢測——電導率法 9.6 生物膜過氧化程度的鑒定——丙二醛含量的測定 9.7 植株組織細胞死亡的鑒定（台盼藍染色法） 9.8 過氧化氫（H2O2）含量的測定 9.9 超氧陰離子產生速率的測定 9.10 超氧化物歧化?（SOD）活性的測定 9.11 過氧化物?（POD）活性的測定 9.12 過氧化氫?（CAT）活性的測定 附錄 附錄1 相關理論知識鞏固與思考 附錄2 化學品標誌符號 附錄3 常用酸鹼的濃度 附錄4 常用固態酸、鹼、鹽的物質的量濃度配製參考 附錄5 常用緩衝液的配製 附錄6 常用酸鹼指示劑 附錄7 標準計量單位 附錄8 常用植物生長物質的一些化學特性 參考文獻 英文篇 Chapter 1 Experimental Basis of Plant Physiology 1.1 Plant physiology laboratory rules 1.2 Classification and preparation methods of chemical reagent 1.3 Collection， processing and preservation of experimental material 1.4 Basic methods of experimental studies on plant physiology Chapter 2 Plant Seed Germination 2.1 Determination of seed germination rate， germination potential and germination index 2.2 Rapid determination of seed viability 2.3 Determination of seed vigor 2.4 The formation and determination of the amylase activity during seed germination 2.5 Changes in fatty acid content of oil seeds during germination 2.6 Changes in amino acid content during seed germination Chapter 3 Plant Water Metabolism 3.1 Determination of plant leaf water content， relative water content and water saturation deficit 3.2 Determination of free and bound water content in plant leaves （Malincic method） 3.3 Determination of plant tissue water potential （Small flow method） 3.4 Determination of plant tissue osmotic potential （Plasma wall separation method） 3.5 Determination of transpiration rate Chapter 4 Effects of Mineral Element Deficiency on Plant Life Activities 4.1 Element deficiency symptoms in plants （solution culture） 4.2 Determination of nitrate reductase activity
4.3 Determination of root activity （α - naphthylamine oxidation method） 4.4 Determination of nitrate nitrogen in plants 4.5 Determination of plant phosphorus （molybdenum blue method） Chapter 5 Evaluation of Plant Photosynthetic Performance 5.1 Extraction and physicochemical properties of photosynthetic pigments 5.2 Separation of photosynthetic pigments and determination of absorption spectra 5.3 Determination of chloroplast pigment content 5.4 Determination of plant leaf photosynthetic rate and gas exchange parameters 5.5 Determination of chlorophyll fluorescence parameters 5.6 Determination of ribulose diphosphate carboxylase （RuBPCase） activity 5.7 Determination of phosphoenolpyruvate carboxylase （PEPCase） activity Chapter 6 Plant Carbon and Nitrogen Metabolism 6.1 Determination of sucrose， glucose and fructose content 6.2 Determination of free amino acid content 6.3 Determination of sucrose synthase and sucrose phosphatase activities 6.4 Determination of α - amylase and β - amylase activities 6.5 Determination of glutamine synthetase activity 6.6 Determination of soluble protein content Chapter 7 The Physiological Effects of Plant Growth Substances and Their Effects on Plant Development 7.1 Effects of auxin on the growth of wheat root and shoot 7.2 Effects of gibberellin on the growth of wheat seedlings 7.3 Effect of ethephon on the fruit ripening 7.4 Abscissive effects of abscisic acid （ABA） on plant petioles 7.5 Effects of gibberellin （GA3） and abscisic acid （ABA） on seed germination 7.6 Synthesis of α - amylase in seed induced by gibberellin （GA3） 7.7 Effects of abscisic acid （ABA） on stomatal movement 7.8 Determination of phytohormones by liquid chromatography Chapter 8 Plant Tissue Culture 8.1 Preparation of culture medium 8.2 Sterilization， disinfection and inoculation 8.3 Subculture and propagation 8.4 Acclimation， transplanting and management of tube seedlings Chapter 9 Plant Stress Physiology 9.1 Determination of proline content 9.2 Determination of total flavonoid content 9.3 Determination of ascorbic acid （AsA， AA） 9.4 Determination of glutathione （GsH） 9.5 Determination of cytoplasmic membrane permeability - conductance method 9.6 Identification of degree of membrance peroxidation - determination of malondialdehyde content （MDA） 9.7 Identification of cell death in plant tissues （Trypan blue staining method） 9.8 Determination of hydrogen peroxide （H2O2） content 9.9 Determina Appendix 5 Preparation of commonly used buffers Appendix 6 Commonly used acid - base indicators Appendix 7 Standard unit of measurement Appendix 8 Some chemical properties of common plant growth substances Bibliography
[