Preface Chapter 1 Introduction 1.1 Conception of Enzyme and Its Development 1.2 The Characteristics of Enzyme Action 1.3 Factors that Affecting Enzyme Action 1.4 Classification and Nomenclature of Enzymes 1.5 Determination of Enzyme Activity 1.6 Methods for Enzymes Production 1.7 Development Survey of Enzyme Engineering Review and Thinking Chapter 2 Enzyme Production by Microbial Fermentation 2.1 Regulation of Enzyme Biosynthesis in Microbial Cells 2.2 Characteristic of Microbes for Enzyme Production 2.3 Fermentation Process Conditions and Its Control 2.4 Enzyme Fermentation Kinetics 2.5 Enzyme Production by Immobilized Cell Fermentation 2.6 Enzyme Production by Immobilized Protoplast Fermentation Review and Thinking Chapter 3 Enzyme Production by Animal and Plant Cell Cultures 3.1 The Regulation of Enzyme Biosynthesis in Animal and Plant Cells 3.2 Enzyme Production by Plant Cell Culture 3.3 Enzyme Production by Animal Cell Culture Review and Thinking Chapter 4 Extraction, Separation and Purification of Enzymes 4.1 Cell Disruption 4.2 Enzyme Extraction 4.3 Precipitation Separation 4.4 Centrifugal Separation 4.5 Filtration and Membrane Separation 4.6 Chromatographic Separation 4.7 Eleetrophoresis Separation 4.8 Extraction Separation 4.9 Crystallization 4.10 Concentration and Drying Review and Thinking Chapter 5 Enzyme Molecular Modification 5.1 Metal Ion Substitution Modification 5.2 Macromolecular Binding Modification 5.3 Side Chain Group Modification 5.4 Peptide Chain Limited Hydrolysis Modification 5.5 Nucleotide Chain Cleavage Modification 5.6 Amino Acid Substitution Modification 5.7 Nucleotide Substitution Modification 5.8 Physical Modification 5.9 Application of Enzyme Molecular Modification Review and Thinking Chapter 6 Immobilization of Enzymes, Cells and Protoplasts 6.1 Enzyme Immobilization 6.2 Cell Immobilization 6.3 Protoplast Immobilization
Review and Thinking Chapter 7 Enzyme Catalysis in Non-aqueous Phases 7.1 The Main Scopes of Enzyme Catalysis in Non-aqueous Phase 7.2 Effects of Water and Organic Solvents on Enzyme Catalysis in Organic Media 7.3 The Catalytic Characters of Enzymes in Organic Media 7.4 Conditions of Enzyme Catalytic Reaction in Organic Media and Its Control 7.5 Application of Enzyme Catalysis in Non-aqueous Phase Review and Thinking Chapter 8 Enzyme Directed Evolution 8.1 The characteristics of Enzyme Directed Evolution 8.2 Random Mutation of Enzyme Gene 8.3 Directed Selection of Enzyme Gene Mutants 8.4 The Applications of Enzyme Directed Evolution Review and Thinking Chapter 9 Enzyme Reactors 9.1 Types of Enzyme Reactors 9.2 Choice of Enzyme Reactors 9.3 Design of Enzyme Reactors 9.4 Enzyme Reactor Operation Review and Thinking Chapter 10 Applications of Enzymes 10.1 Enzymes Application in Pharmaceutical Industry 10.2 Application of Enzymes in Food 10.3 Enzyme Application in Light and Chemical Industries 10.4 Enzyme Application in Environmental Protection 10.5 Enzyme Application in Biotechnology Review and Thinking Main references
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