內容大鋼
本書較為系統總結和歸納了結縷草屬植物種質資源相關的基礎理論和應用技術，重點針對結縷草實際應用中的熱點和難點問題展開論述。全書共分為四章。第一章為緒論，主要介紹結縷草資源的基本概況，包括結縷草屬植物的起源、分佈、分類情況，種子和基因組特性，應用現狀以及面臨的機遇與挑戰；第二章主要以結縷草種子建植過程中存在的休眠、機械化精量播種困難、苗期生長緩慢及雜草防控等問題為切入點，分四節來分別介紹穎引髮結縷草種子休眠機理以及不同類型的結縷草種子丸粒化包衣技術；第三章為基於分子標記的結縷草遺傳學研究，如結縷草高密度分子標記遺傳連鎖圖譜、結縷草高密度整合遺傳圖譜、基於結縷草與水稻同源性的比較基因組、結縷草品種的DNA指紋圖譜；第四章則由五節組成，針對影響結縷草草坪應用和質量的最常見病害——鏽病，以資源收集與鏽病評價為起點，圍繞結縷草種質資源多地多點鏽病抗性聯合分析、結縷草鏽病病原菌的分離與鑒定、病原菌浸染進程分析、結縷草抗感鏽病種質的葉片組織結構差異分析、抗氧化系統響應、鏽病抗性關鍵基因篩選等，通過不同角度，表型、生理和分子不同水平，較為全面的解析和闡述結縷草鏽病響應機制。本書為英文版。
目錄
1 Introduction
1.1 Origin and distribution of Zoysia
1.2 Genome and seed characteristics
1.3 Application
1.4 Opportunities and challengesReferences
2 Research on the seed dormancy mechanism and seed coating technology of zoysiagrass
2.1 Different seed dormancy levels imposed by tissuescovering the caryopsis in zoysiagrass (Zoysia japonica)
2.1.1 Preliminary germination tests
2.1.2 Scanning electron microscopy (SEM) analysis
2.1.3 Effects of cut-seed treatments on germination
2.1.4 Water uptake rate
2.1.5 Effect of light on germination
2.1.6 Discussion
2.1.7 Conclusions
2.2 Research on conventional seed coating technology for zoysiagrass
2.2.1 The quality test results of seed pellets
2.2.2 Results of seed germination on paper beds
2.2.3 Results of seed germination on sand and soil beds
2.2.4 Discussion
2.2.5 Conclusions
2.3 Research on weed-resistant seed coating technology for zoysiagrass
2.3.1 Effectiveness of weed control
2.3.2 Coated seed quality testing
2.3.3 Discussion and conclusions
2.4 Research on saline-tolerant seed coating technology for zoysiagrass
2.4.1 Effect of different concentrations of saline and alkaline stress on seed germination of zoysiagrass
2.4.2 Effects of soaking seeds with four kinds of exogenous substances on seed germination under salt-alkali stress
2.4.3 Preparation and design of saline–alkali tolerant pelleted seeds
2.4.4 Quality test results of saline-alkali tolerant pelleted seeds
2.4.5 Germination results on paper of saline–alkali tolerant pelleted seeds
2.4.6 Sand bed test results of saline-alkali tolerant pelleted seeds
2.4.7 Soil bed test results of saline-alkali tolerant pelleted seeds
2.4.8 Comparison of production costs of saline–alkali tolerant pelleted seeds
2.4.9 Discussion
References
3 Genetics research of zoysiagrass based on molecular marker technology
3.1 Construction of a high-density simple sequence repeat marker-based linkagemap of zoysiagrass (Zoysia japonica)
3.1.1 Marker screening and marker segregation
3.1.2 Map construction
3.1.3 Comparison between the AFLP-SSR map and the high-density SSR map
3.1.4 Synteny between zoysiagrass and rice
3.1.5 Genomic constitution of Zoysia species
3.2 An integrated SSR based linkage map for Zoysia matrella and Zoysia japonica
3.2.1 Marker screening and marker segregation
3.2.2 Genetic linkage map of Zoysia matrella
3.2.3 Comparison of the Zoysia matrella map with two SSR based linkage maps for Zoysia japonica
3.2.4
3.3.2 New linkage map based on SSR, RFLP, CISP and PLUG markers
3.3.3 Synteny between zoysiagrass and rice
3.3.4 Conclusions
3.4 Construction of SSR based fingerprint map of Zoysia varieties
3.4.1 Selection and determination of core primers
3.4.2 Construction of fingerprint maps of Zoysia varieties
3.4.3 Discussion and conclusions
References
4 Evaluation and response mechanism of rust resistance in zoysiagrass
4.1 Joint test analysis on rust resistance of zoysiagrass germplasm resources atJiaozhou, Qingdao and Yangzhou, Jiangsu in 2010 and 2011
4.1.1 Observation results at Yangzhou, Jiangsu Province in 2011
4.1.2 Observation results at Jiaozhou, Shandong Province in 2010 and 2011
4.1.3 Joint test of three observations at the two sites
4.1.4 Discussion and conclusions
4.2 Evaluation of rust resistance of zoysiagrass germplasm resources for consecutive years from 2011 to 2013 at Yangzhou Jiangsu Province
4.2.1 Field incidence survey of zoysiagrass rust
4.2.2 Evaluation results of rust resistance and susceptibility types inzoysiagrass
4.2.3 Discussion and conclusions
4.3 Analysis of leaf tissue structures between rust resistant and rust susceptible zoysiagrass (Zoysia japonica)
4.3.1 The wax content of rust susceptible and rust resistant zoysiagrass
4.3.2 The stomata number of rust susceptible and rust resistant zoysiagrass
4.3.3 Leaf structures of rust susceptible and rust resistant zoysiagrass
4.3.4 Discussion and conclusions
4.4 Isolation and identification rust pathogens and the study of antioxidant enzyme activity and gene expression under rust infection in Zoysia japonica
4.4.1 Morphological identification of zoysiagrass rust pathogen
4.4.2 Sequence homology analysis of zoysiagrass rust pathogen 18S rDNA
4.4.3 The disease severity and infection type survey
4.4.4 Morphological observations of zoysiagrass leaves inoculated with rust pathogen
4.4.5 Antioxidant enzyme activity
4.4.6 Gene expression analysis
4.4.7 Discussion and conclusions
4.5 Physiological and molecular responses of Zoysia japonica to rust infection
4.5.1 Whole-plant observations of Zoysia japonica leaves after inoculation
4.5.2 Cellular observations of Zoysia japonica leaves after inoculation
4.5.3 Electrolyte leakage (EL) and chlorophyll content (Chl)
4.5.4 Transcriptome analysis
4.5.5 Leaf cell structure and physiological changes after inoculation withrust pathogen
4.5.6 Transcriptomic response to inoculation with rust pathogen
4.5.7 Conclusions
References
Supplemental Tables
Supplemental Figures
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