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再生醫學和藥物基因輸送實驗手冊(來華留學生英文授課精編教材)(英文版)

  • 作者:編者:曹霞|責編:仲蕙
  • 出版社:江蘇大學
  • ISBN:9787568418720
  • 出版日期:2022/12/01
  • 裝幀:平裝
  • 頁數:95
人民幣:RMB 35 元      售價:
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內容大鋼
    本書包括20個再生藥物及傳遞常用實驗項目,首先介紹了再生醫學的實驗場地要求,其次介紹了組織工程構建時種子細胞的分離和培養,重點介紹原代細胞分離、純化和培養的方法,之後重點介紹了幾種常用的藥物傳遞系統的構建,包括納米粒、脂質體、微囊等,同時介紹了幾種常用的組織工程支架構建方法,此外,結合最新3D列印技術,介紹同軸列印的方法構建組織工程化血管,最後介紹了幾種常見再生藥物及傳遞的儀器使用方法。本書的出版能幫助留學生更好地進行再生醫學和藥物、基因輸送相關實驗,對提高留學生教學質量及來華留學生課程建設具有積極意義。

作者介紹
編者:曹霞|責編:仲蕙

目錄
Chapter 1 Establishment of Tissue Engineering Laboratory
  Laboratory design for cell culture
  Rules and regulations for cell culture room
Chapter 2 Seed Cell Culture and Identification
  Basic principles of in vitro cell culture
  Experiment 1 Isolation and culture assay of primary cells
  Experiment 2 Primary cell subculture and its passage assay
  Experiment 3 Freezing and resuscitation of cells
  Experiment 4 MTT method to measure cell proliferation rate
  Experiment 5 Immunostaining assay
  Experiment 6 DAPI nuclear fluorescent labeling assay
  Experiment 7 Cell migration assay
  Experiment 8 SDS-polyacrylamide gel electrophoresis assay
  Experiment 9 Isolation and cultivation of rat bone marrow mesenchymal stem cells
Chapter 3 Fabrication of Three-dimensional Scaffolds
  Experiment 10 Solvent casting assay
  Experiment 11 Gel pressing method assay
  Experiment 12 Preparation of injectable thermosensitive hydrogel
  Experiment 13 Freeze-drying method assay
  Experiment 14 Three-dimensional tissue printing technique assay
  Experiment 15 Coaxial bioprinting of dual network bioink to prepare blood vessels
Chapter 4 Growth Factor Delivery Systems for Regenerative Medicine
  Experiment 16 Preparation of hydrogel for growth factor delivery
  Experiment 17 Application of hydrophobic polymers in GF transport
  Experiment 18 Growth factor perfusion delivery assay
  Experiment 19 pH sensitive drug delivery assay
  Experiment 20 Magnetically responsive drug delivery assay
Chapter 5 Use of Common Instruments and Equipment for Tissue
  Engineering
  Workbench
  Inverted fluorescence microscope
  Carbon dioxide (CO2) incubator
  Bioreactor
  Laser confocal microscopy
References

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