Chapter 1 Reactive Platforms for Controllable Fabrication of Functional (Bio)Interfaces 1.1 Scope of Surface Modification for (Bio)Reactive Platfor ms and Functional (Bio)Interfaces 1.2 Chemical and Topographical Pattern Fabrication from Micrometer to Nanometer Length Scales References Chapter 2 Surface Reactions and Fabrication of Bioreactive Platforms Based on Organic and Polymeric Films: From Micrometer to Nanometer Length Scale 2.1 Introduction: Bioreactive Platforms and Biointerfaces via Organic and Polymeric Films 2.1.1 Organic and Polymeric Films 2.1.2 Pattern Fabrication on Organic and Polymeric Films 2.2 Surface Reactions of Organic and Polymeric Films 2.2.1 Self-assembled Monolayers 2.2.2 Surface Modification with Polymers 2.3 Patterned Organic and Polymeric Films for Tailored (Bio)Interfaces 2.3.1 Microcontact Printing 2.3.2 Selective Molecular Assembly Patterning (SMAP) 2.3.3 Embossing and Nano-Imprinting 2.3.4 Self-assembled Block Copoly mer Patterns References Chapter 3 Confinement Effects on the Reactivity in Ultrathin Polymer Films: Kinetics and Temperature Dependence of the Hydrolysis of NHS and TBA Esters 3.1 Introduction 3.2 Ultrathin PNHSMA Polymer Films 3.2.1 Characterization of the Hydrolysis of PNHSMA Films 3.2.2 Temperature Dependence of Reaction Kinetics 3.2.3 Comparison of Surface Reactions in SAMs and Ultrathin Polymer Films 3.3 Ultrathin PS_-b-PiBA_ Polvmer Films 3.3.1 Characterization of Polymer Thin Film Surface Composition 3.3.2 Characterization and Kinetics of the Hydrolysis of PSm-b-PtBA, Films 3.3.3 Investigation of the Temperature Dependence of Reaction Kinetics 3.3.4 Effects of Film Thickness and Thermal Pretreatment on Reaction Rates 3.3.5 Dependence of Hydrolysis Kinetics on Surface Composition 3.3.6 Comparison of Reactivity of T-Butyl Esters in PS,-b-PtBAm Thin Films and in Solution 3.4 Experimental Section References Chapter 4 Reactive Thin Polymer Films as Platfor ms for the Immobilization of Biomolecules
4.1 Introduction 4.2 Investigation of the Reactivity of PNHS MA 4.2.1 Coupling of PEGsoo-NH2 4.2.2 Coupling of Fluoresceinamine and BSA to PNHSMA Films1wvve 4.2.3 Comparison of Surface Reactions on PNHSMA Films and NHS-C10 Monolayers 4.3 Swelling Behavior and Stability of PNHSMA Films 4.4 Immobilization of DNA and Surface-Based Hybridization 4.4.1 DNA Immobilization Studied by SPR 4.4.2 Investigation of DNA Hybridization by SPFS and Fluorescence Microscopy 4.5 Toward the Detection of Pathogenic Bacteria on PNHSMA Films 4.6 Experimental Section References Chapter 5 Tailored Biointerfaces via Derivatization of Polystyrene-b-Poly(Tert-Butyl Acrylate) Thin Films 5.1 Introduction 5.2 Investigation of the Surface Chemistry of PS690-b-PtBA1210 Films 5.2.1 Surface Hydrolysis of PS690-b-PtBA1210 Films 5.2.2 Covalent Coupling of PEG to Activated PSkon-b-PtBA n Films 5.3 Covalent Coupling of Biomolecules to Activated PS690-b-PtBA1210 Films 5.4 Cell Adhesion Studies on PSs90-b-PtBA1210 Films 5.5 Experimental Section References Chapter 6 Fabrication of Robust Biomolecular Patterns by Reactive Microcontact Printing on NHS Ester Containing Polymer Films 6.1 Introduction 6.2 Approaches for Micropatterning of PNHSMA Films 6.3 Functionalization of PNHSMA Films by Reactive u.CP 6.4 Non-specific Adsorption (NSA) 6.5 Patterning of PNHSMA and Coupling of (Bio)Molecules to Patterned Films 6.6 DNA Hybridization 6.7 Experimental Section References Chapter 7 Reactive uCP on Ultrathin Block Copolymer Films Investigation of the uCP Mechanism and Applications to Sub-um (Bio)Molecular Patterning 7.1 Introduction 7.2 Strategy A: Fabrication of Multimolecular Arrays by Direct Molecular Transfer 7.3 Strategy B: Local Passivation of PS690-b-PtBA1210 Films 7.4 Strategy C: Local Deprotection of PS90-b-PtBA1210 Films 7.4.1 Analysis by Fluorescence Microscopy 7.4.2 Analysis by AFM
7.4.3 Mechanism of Local Hydrolysis 7.4.4 Local Deprotection on the Sub-um Scale 7.5 Experimental Section References Chapter 8 Nanofabrication on Reactive Block Copolymer Film Platforms Toward Microdomain-Selective Chemical Functionalization 8.1 Introduction 8.2 Solvent-Induced Surface Reorientation of PS-b-PtBA Films 8.3 Domain-Selective Coupling on Solvent-Treated Polymer Films 8.3.1 Global Characterization of Domain-Selective Coupling 8.3.2 Characterization of Do main-Selective Coupling at the Nanometer Level 8.4 Area-Selective Functionalization of Block Copolymer-Based Nanopatterned Platforms 8.5 Outlook 8.6 Experimental Section References Chapter 9 Bioinspired Hierarchically Structured Poly mer Interfaces for Prornising Biomedical Applications 9.1 Introduction 9.2 Preparation of Rose Petal-Derived PDMS Surface Functionalized with Anti-EpCAM 9.2.1 Fabrication of Hierarchically Structured PDMS 9.2.2 Chemical Modification of Anti-EpCAM on Hierarchically Structured PDMS Surface 9.3 Cell-Capture on Functionalized Hierarchical PDMS 9.3.1 Cell-Capture 9.3.2 Cell Release 9.4 Conclusions and Outlook 9.5 Experimental Section References
[