內容大鋼
王躍春主編的《生理學實驗指南及醫學研究基礎》根據作者多年的教學經驗和實踐編寫而成。為適應功能實驗室平台的建設,本書介紹了學生進行觀察和掌握的核心實驗內容,對兩種常規使用的軟體系統(BL-420i integrated Signal acquisition and processing system 和Webchat system 4000 for human experiments)進行詳細的介紹;為了順應建設高水平大學和培養具有科研思維和創新精神的複合型人才的需要,增加了實驗報告和科研論文的書寫規範與技巧、生理學實驗設計、基本醫學科研方法。為實現高等教育要提高學生人文素養和創新意識的目標,書中還增加了與「諾貝爾生理或醫學獎」相關的生理學經典實驗的介紹等內容。
本書能反映學科最新教學、科研成果和時代特色,且滿足我校建設高水平大學的人才培養目標的需要;內容有創新性和實踐性特點,符合學生的知識基礎、心理特點和認識規律。
作者介紹
編者:王躍春
王躍春,血液內科學博士,暨南大學基礎醫學院生理學系副教授,碩士生導師,主要研究方向為神經生理與臨床以及幹細胞生理與臨床。2010—2012年在香港大學李嘉誠醫學院外科學系、2013—2015年在美國加州羅馬琳達大學生理學系及神經生物學研究室做訪問學者。廣東省「生理學精品課程」「雙語生理學網路課程」「廣東省生理學教學團隊項目」以及教育部「來華留學優質示範課程生理學」的核心成員。在教學方面,主持完成「人體生理學」和「諾貝爾獎解析一醫學篇」兩項優質示範課程,廣受好評;開設通識教育核心課程「諾貝爾獎解析一醫學篇」。以第一作者發表SCI論文8篇。主持各類科研課題8項。編寫出版《感覺器官生理學》《環境生理學》、ClinicalRespiratory Medicine, Peptide Nanofiber Scaffold forBrain Tissue Reconstruction等多本著作和教材。
目錄
序
Preface
Chapter I Introduction to Physiological Experiments
Section 1 General course description of physiological experiments
Section 2 Laboratory rules and requirements of physiological experiments
Section 3 The development of physiological experiment and basic types of physiological experiment
Section 4 Preparation for common reagents used in physiological laboratory
Section 5 Formats and requirements of physiological experimental reports
Chapter II Animal Experiments
Section 1 Basic technique for amphibian animal experiments
Section 2 Basic technique for mammal experiments
Section 3 Basic technique for rodent animal experiments
Section 4 BL- 420 biological data acquisition and analysis system
Section 5 Specific animal experiments
Experiment 1 Sciatic-gastrocnemius preparation
Experiment 2 Threshold, supra-threshold and maximal stimulus
Experiment 3 Single muscle twitch, frequency summation, and tetanization
Experiment 4 Action potential of nerve trunk
Experiment 5 Conduction velocity of compound AP of nerve trunk
Experiment 6 The pacemaker of frog heart
Experiment 7 Extrasystole and compensatory pause of the frog heart
Experiment 8 Osmotic fragility of the red blood corpuscles
Experiment 9 Regulation of respiration movement
Experiment 10 Regulation of cardiovascular system
Experiment 11 Physiological properties of smooth muscle of digestive tract
Experiment 12 Factors affecting urinary formation
Experiment 13 The reflex arc
Experiment 14 Ataxia induced by cerebellar injury in mouse
Chapter III Human Experiments
Section I Introduction to WebChart -400 system for human experiments
Section 2 Specific human experiments
Experiment 1 Recording of human respiratory movement
Experiment 2 Detection of heart sounds
Experiment 3 Measurement of blood pressure
Experiment 4 Recording of an electrocardiogram
Experiment 5 Determination of ABO blood types
Experiment 6 Examination of visual acuity
Experiment 7 Examination of visual fields
Experiment 8 Examination of blind spot
Experiment 9 Examination of color blindness
Experiment 10 Conduction ways of sound
Experiment 11 Induction of knee jerk reflex
Experiment 12 Human auditory and visual responses
Chapter IV Fundamentals of Medical Research
Section 1 Scientific method and experimental medicine
Section 2 Basic principles of experimental medicine
Section 3 Guidelines to write and publish a research paper
Chapter V Experimental Design
Section 1 Introduction to experimental design
Section 2 Methods for designing a g
Section 3 An example of physiological experimental design
Section 4 An example of a full lab report
Section 5 Suggested topics for physiological experimental design
Appendix
Appendix 1 Laboratory syllabus for physiological experiment(Applicable to clinical medicine)
Appendix 2 Laboratory syllabus for human physiological experiment(Applicable to phamacology)
Appendix 3 Grade table for examination in physiological experiments
Appendix 4 An example of physiological experimental report
Acknowlegement
鳴謝