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番茄果實成熟中的果膠甲酯?(英文版)

  • 作者:溫波//朱世東//(英)塔克
  • 出版社:中國農業科學技術
  • ISBN:9787511624116
  • 出版日期:2016/01/01
  • 裝幀:平裝
  • 頁數:132
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內容大鋼
    溫波、朱世東、塔克所著的《番茄果實成熟中的果膠甲酯?(英文版)》主要介紹作者在英國研究期間從事園藝植物番茄生理生化、遺傳育種、果實采后貯藏加工等生物學的研究與實驗等方面所取得的新成果。

作者介紹
溫波//朱世東//(英)塔克

目錄
CHAPTER 1 INTRODUCTION
  1.1 Fruit
    1.1.1 General introduction
    1.1.2 Tomato
  1.2 Fruit ripening
    1.2.1 Colour, flavour and texture changes in fruit ripening
    1.2.2 Ethylene in fruit ripening
  1.3 Plant cell wall
    1.3.1 General introduction
    1.3.2 Cell wall components
    1.3.3 Cell wall structure
  1.4 Cell wall enzymes
    1.4.1 Polygalacturonase (PG)
    1.4.2 Pectate lyases
    1.4.3 β-Galactosidase
    1.4.4 Expansin
  1.5 Pectinmethylesterase
    1.5.1 PE proteins
    1.5.2 PE modes of action and regulation
    1.5.3 PE genes and isoforms
    1 5.4 Tomato PE antisense lines
CHAPTER 2 MATERIALS AND METHODS
  2.1 Plant material
    2.1.1 Tomato growth and maintenance
    2.1.2 Crossing tomato
  2.2 Chemicals
  2.3 Fruit texture analysis
  2.4 Biochemical methods
    2.4.1 Extraction of crude protein from tomato fruits
    2.4.2 Bio-Rad (Bradford) protein assay
    2.4.3 Pectinesterase assay
    2.4.4 Heparin affinity chromatography
    2.4.5 PE profile assay
    2.4.6 Concentration of pooled PE isoforms
    2.4.7 Total leaf protein extraction for SDS PAGE analysis.
    2.4.8 Making acetone insoluble solid (AIS)
    2.4.9 Determination of degree of esterification (DE) of the pectin by titration
    2.4.10 Extraction of CDTA Soluble Pectin
    2.4.11 Determination of Neutral Sugars
    2.4.12 Determination of uronic acid
  2.5 Molecular biological methods
    2.5.1 DNA extraction from tomato leaves
    2.5.2 DNA measurement
    2.5.3 Polymerase chain reaction (PCR)
    2.5.4 Restriction enzyme digestion
    2.5.5 Agarose gel electrophoresis
    2.5.6 DNA sequence (MWG value read)
    2.5.7 PCR product purification by Perfectprep Gel Cleanup Kit (Eppendorf):
  2.6 Immunochemistry and immunolabelling
    2.6.1 Immunodot assay

    2.6.2 Immunolocalization
  2.7 Statistics
  2.8 Capillary Electrophoresis (CE)
    2.8.1 Enzyme digests
    2.8.2 CE
CHAPTER 3 GENERATION OF DOUBLE ANTISENSE PLANTS
  3.1 Plant generation
  3.2 Generation of the double antisense plants
  3.3 PE Isoform profilers of double antisense plants
    3.3.1 PE extraction method optimisation
    3.3.2 PE profiles in tomato fruit
  3.4 Summary
CHAPTER 4 CHARACTERIZATION OF PE SINGLE AND DOUBLE ANTISENSE PLANTS
  4.1 Fruit PE activity,and morphology analysis
    4.1.1 Generation of wild-type controls
    4.1.2 Fruit PE activities
    4.1.3 Fruit morphology and softening
  4.2 Cell wall component analysis
    4.2.1 UA in acetone insoluble solids (AIS) and CDTA soluble pectin
    4.2.2 DE of pectin in AIS
    4.2.3 Neutral sugar analysis by GC
  4.3 Capillary electrophoresis (CE) of pectin
    4.3.1 DE of CDTA soluble pectin
    4.3.2 CE profiles of PG digested CDTA soluble pectin
  4.4 Characterization of antisense lines using monodonal antibodies
    4.4.1 Immuno-dot blot analysis of CDTA soluble pectin
    4.4.2 Immunolocalization: Fruit pericarp
    4.4.3 Immunolocalization: Stem
  4.5 Summary
    4.5.1 Biochemical and morphological study
    4.5.2 Immunological study
CHAPTER 5 USE OF ANTISENSE LINES FOR PE ISOFORMS ANALYSIS
  5.1 Introduction
  5.2 Effect of pH on PE activity
  5.3 PE isoform profiling
    5.3.1 Fruit
    5.3.2 Stem
    5.3.3 Leaf
  5.4 Summary
CHAPTER 6 OVERALL DISCUSSION
  6.1 Role of PE during fruit ripening
  6.2 PE isoforms in vegetative tissues
  6.3 Unstable PE1 expression in double antisense lines.
  6.4 Future work
REFERENCES

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