內容大鋼
本書難易適中、內容創新實用，主要適用對象為醫學留學生（本科及研究生），以及我國醫學長學制碩博研究生。本書在上一版基礎上增加了分子生物前沿技術發展及在醫學研究中現代常用技術的內容；對生物化學實驗技術基礎理論與經典實驗部分進行了修訂，介紹基本操作技術、分光光度法、電泳技術、層析技術的原理及經典實驗，並以核酸、蛋白質為對象，介紹研究基礎實驗的原理和應用；凝練了綜合性實驗實踐，以?學分析、蛋白質分離純化鑒定、核酸分離及鑒定、基因工程為系統性實驗內容；新增了醫學創新實驗設計與應用，以生物大分子的結構與功能研究為對象，結合醫學學科研究特點，提出引導問題，為學生開展自主創新實驗的設計與應用提供設計流程、模擬報告的指導。
目錄
Section Ⅰ Theories and Strategies of Biochemistry and
Molecular Biology Technology in Medical Research
Chapter 1 Medical Application of Biochemistry and Molecular Biology Technology
1.1 Basic Objectives of Biochemistry and Molecular Biology Technology
1.2 Application of Biochemistry and Molecular Biology Technology in Medical Research
Chapter 2 The Common Research Techniques of Gene
2.1 Nucleic Acid Hybridization and DNA Microarray
2.2 DNA Sequencing
2.3 Polymerase Chain Reaction (PCR)
2.4 Recombinant DNA Technology
2.5 The Research Strategies and Methods for Gene Transcription Regulation
Chapter 3 The Common Research Techniques of Protein
3.1 Quantification and Identification of Protein
3.2 Methods and Basic Principles of Protein Purification
3.3 Protein Chemical Modification and Spatial Structure Analysis
3.4 Protein Interaction Research Methods
3.5 Proteomies Research
3.6 Research Technology of Enzymology
Section Ⅱ Basic Biochemical Techniques
Chapter 4 Basic Operation of Biochemical Experiments
4.1 Solution Preparation
4.2 Pipettes Operation
4.3 Centrifuge Operation
Chapter 5 Spectrophotometry
5.1 Basic Concepts and Principle of Spectrophotometry
5.2 Measurement of Light Absorption
5.3 Applications of Spectrophotometry
Experiment 1 Quantitative Analysis of Protein
Experiment 2 Determination of Blood Glucose Level Regulated by Hormones
Chapter 6 Electrophoresis
6.1 Basic Principles of Electrophoresis
6.2 Impact Factors of Electrophoresis
6.3 Detection of Components after Electrophoretic Separation
6.4 Special Electrophoretic Techniques and Applications
Experiment 3 Separation of Serum Proteins by Cellulose Acetate Membrane Electrophoresis
Experiment 4 Separation of Serum Lipoproteins by Agarose Gel Electrophoresis
Experiment 5 Proteins Separation by SDS-Polyacrylamide Gel Electrophoresis
Chapter 7 Chromatography
7.1 Fundamental Principles of Chromatography
7.2 Commonly Used Chromatographic Techniques
Experiment 6 Separation of Mixed Amino Acids by Cation Exchange Chromatography
Experiment 7 Separation of Hemoglobin and Dinitrophenyl (DNP) -glutamate by Gel Filtration Chromatography
Section Ⅲ Integrative Experiments
Chapter 8 Enzyme Analysis
8.1 The Activity of Enzyme
8.2 Enzyme Kinetics ( Factors Affecting Reaction Velocity)
8.3 Enzymatic Analysis
Experiment 8 Assay the Activity of Alanine Aminotransferase (ALT) in Serum ( Mohun's Method)
Experiment 9 Effect of Substrate Concentration on Enzyme Activity-Determining Km Value for Alkaline Phosphatase
Experiment 10 Enzymatic Endpoint Method-Determination of Total Cholesterol, Triglycerides and Glucose Levels in Serum
Chapter 9 Isolation, Purification and Identification of Protein
9.1 Procedure of Protein Isolation
9.2 The Selective Purification Steps
9.3 Crystallization, Concentration, Drying and Storage of Protein
9.4 Identification and Analysis of Protein
Experiment 11 Isolation and Identification of Serum IgG
Experiment 12 Purification and Identification of Glutathione S-Transferase Fusion Protein
Chapter 10 Isolation, Purification and Identification of Nucleic Acids
10.1 Preparation Principle and Procedure of Nucleic Acids
10.2 Identification and Analysis of Nucleic Acids
Experiment 13 Isolation and Identification of Eukaryotic Genomie DNA
Experiment 14 SDS-Alkaline Lysis Preparation of Plasmid DNA
Experiment 15 Isolation and Identification of Total RNA in Tissues/Cells
Experiment 16 Polymerase Chain Reaction (PCR) and Reverse Transeription-PCR
Chapter 11 Genetic Engineering
11.1 The Process of Genetic Engineering
11.2 Expression of Foreign Genes and Purification of Recombinant Proteins
Experiment 17 DNA Cloning
Experiment 18 Expression of Exogenous Gene in E.eoli and Purification of His-tag Proteins
Chapter 12 Innovation Experiment Design and Application
Experiment 19 Separation and Identification of Alkaline Phosphatase
Experiment 20 Lactate Dehydrogenase (LDH) Analysis
Experiment 21 Protein Markers Exploration by Two-dimensional Eleetrophoresis
Reference
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