前言 實驗1 光學顯微鏡的使用及細胞周期 實驗2 細胞傳代培養及細胞融合 實驗3 骨髓間充質幹細胞原代培養與定向分化 實驗4 人染色體核型分析 實驗5 分光光度法(雙縮?法、BCA法和考馬斯亮藍法測定蛋白質含量) 實驗5(1) 雙縮?(Biuret)法測定蛋白質含量 實驗5(2) BCA法測定蛋白質含量 實驗5(3) 考馬斯亮藍法(Bradford)測定蛋白質含量 實驗6 層析法 實驗6(1) 凝膠層析分離血紅蛋白與魚精蛋白 實驗6(2) 離子交換層析分離混合氨基酸 實驗7 鹼性磷酸?米氏常數的測定 實驗8 離心法—適應性免疫反應——淋巴細胞分離及鑒定 實驗8(1) 單個核細胞的分離——密度梯度離心法 實驗8(2) 淋巴細胞的鑒定——B淋巴細胞數量檢測 實驗9 電泳法—SDS—聚丙烯?胺凝膠電泳分離混合蛋白質 實驗10 正常和糖尿病大鼠血糖和血脂的檢測及激素對血糖的影響 實驗11 人頰黏膜上皮細胞基因組DNA的抽提及ACE基因多態性的檢測 Preface Experiment 1 Optical Microscope and Cell Cycle Experiment 2 Cell Culture and Cell Fusion Experiment 3 Primary Culture and Directional Differentiation of Rat Bone Marrow Mesenchymal Stem Cells Experiment 4 Human Karyotype Analysis Experiment 5 Spectrophotometry and Determination of Protein Concentration Experiment 5(1) Biuret Assay Experiment 5(2) BCA Assay Experiment 5(3) Bradford Assay Experiment 6 Chromatography Experiment 6(1) Using Gel Filtration Column Chromatography to Separate Hemoglobin and Nucleoprotamine Experiment 6(2) Separating Amino Acids Mixture by Ion-Exchange Chromatography Experiment 7 Determination of Km of Alkaline Phosphatase Experiment 8 Centrifugation Technique --Adaptive Immune Response: Isolation and Identification of Lymphocyte Experiment 8(1) The Separation of Monocytes by Density Gradient Centrifugation Experiment 8(2) Lymphocyte Identification--Counting the Number of B Lymphocyte Experiment 9 Polyacrylamide Gel Electrophoresis of Proteins Experiment 10 Effect of Hormone on Blood Sugar and Lipoprotein of Normal and Diabetic Rat Experiment 11 Polymorphism Analysis of ACE Gene by Extracting Genome DNA of Buccal Epithelial Cells Reference
[